CBI Field Sites

If you would like access to one of the CBI field site, please complete the request form below. You will receive an email with approval or denial in the next 7 days. Without approval, you should not proceed with your sampling trip.   Requests are typically declined due to conflicts of schedule, sampling approach already requested by others, or the field is unavailable.

 

Field Site Access Request Form   

Poplar Field Sites

 

Clatskanie – GWAS

Managed by Poplar Innovations

Brian Stanton – brian.stanton@poplarinnovations.com

The Genome Wide Association Study (GWAS) is CBI’s foundational P. trichocarpa mapping population.  It was assembled from ~1,200 fully re-sequenced individuals representing a latitudinal gradient ranging from 38.8° to 54.3°N.  The Clatskanie GWAS study is the oldest of four replicated trials at other locations.  The purpose is to overcome barriers to gene discovery, validation, and deployment using GWAS breeding selections to identify causal mutations underlying natural variations in cell wall composition and biomass yield.  Selected P. trichocarpa genotypes are being hybridized both intra- and inter-specifically using targeted genomic selections for breeding elite poplar feedstocks. Genomic selection algorithms are being developed to predict the performance of the inter-specific hybrids of P. trichocarpa and P. deltoides that will be verified in field assessments of elite families and clonal progeny.

 

Corvallis – GWAS

Managed by Oregon State University

Jared Leboldus – jared.leboldus@science.oregonstate.edu

Seven acres of agricultural land was planted with approximately 4,000 cuttings of P. trichocarpa in 2024. The tree genotypes are planted in triplicate. These trees are sequenced and part of a GWAS study to identify genes that control traits associated with wood characteristics, growth, disease resistance, and abiotic stress response.

 

University of California-Davis – GWAS

Gail Taylor – gtaylor@ucdavis.edu

The genomewide association study (GWAS) experiment consists of a set of unique 1100 genotypes collected from across a latitudinal gradient in the western USA, with contrasting rainfall. The site is 15 acres, drip irrigated and fertigated, and designed to impose a controlled drought treatment across half of the site with three replicates of each genotype fully irrigated and three subjected to a reduced irrigation treatment, imposing a soil moisture deficit of up to 0.15 MPa each year. The site is instrumented with soil tensiometers and volumetric measurements of soil moisture. A weather station is located on the farm. The trees were planted April 2020, cut back and coppiced in 2023 and reduced to a single stem in 2024.

 

University of California-Davis – Transgenic

Gail Taylor – gtaylor@ucdavis.edu

One of three transgenic field sites, this site consists of a set of unique genotypes of poplar 717, gene edited, to investigate long-term field performance of modified traits identified from the GWAS pipeline, including traits for tree productivity and performance and those associated with cell wall traits for processing and SAF production. The site is less than 1 acre, fully irrigated and fertigated, with weather station nearby. Tensiometers are deployed to follow soil moisture content.

 

University of Maryland Eastern Shore (UMES) – Transgenic

Jonathan Cumming – jrcumming@umes.edu

The UMES poplar field site was established in 2021 as an eastern site for poplar biomass feedstock production trials. The first experiment in was a P. deltoides planting to generate seasonal growth patterns, biomass accretion (above and belowground), and changes in soil properties, including carbon accretion and enzymes associated with nutrient cycling.

In 2024, we established an additional planting of transgenic 717 poplar lines (one of three transgenic field sites) as part of the MD-CA-OR transgenic trial. At the MD site, we will be assessing a suite of variables reflecting growth and performance, with a focus here on root allocation and carbon sequestration in soils.

 

Westport – QTL Population

Managed by Poplar Innovations

Brian Stanton – brian.stanton@poplarinnovations.com

The objective of this study is to identify and map loci controlling phenotypic variation in biomass, wood composition, Melampsora rust resistance, Venturia shoot blight, carbon partitioning, and interaction with the soil microbiome in an inter-specific pseudo-backcross family (identity 52-124).  The backcross taxon — [P. trichocarpa × P. deltoides] × P. deltoides — is composed of approximately 350 BC1 genotypes that is being managed in a randomized complete block design of three replicates in field one of Poplar Innovations’ Westport Tree Improvement Center.  (The backcross was made to P. deltoides ′D124′ in place of the original P. deltoides parent of the F1 family (P. deltoides ′IL-101′) to avoid inbreeding depression.)   The study continues 2016 experimentation with family 52-124 at Poplar Innovations’ Boardman Research Site.

Switchgrass Field Sites

 

Ironhorse Farm – GWAS

Managed by University of Georgia

Thomas Pendergast – thpiv@uga.edu

Two replicated blocks of a 330 genotypes switchgrass GWAS panel using a randomized honeycomb design with 3m between linear plants and weedcloth.  Block 1 was established in May 2019; Block 2 established in July 2020.

 

Tifton – Breeding Population

Managed by University of Georgia

Ali Missaoui – cssamm@uga.edu
A panel of 200 half-sib families generated from polycrosses of 50 parents selected from the GWAS panel evaluated for NUE, drought, and biomass yield and composition will be planted at 3 locations in GA and TN. We are seeking more environments in other locations (North and West). Four genotypes from each family will be planted in replicated rows ( 800 genotypes per rep). Biomass yield and composition traits will be measured for 3 years. The phenotypic data will be used for validation of genomic breeding value estimates and selection of accurate genomic prediction models that facilitate the selection of best genotypes in biomass yield and composition and increase the efficiency of favorable alleles stacking.

 

Tifton – GWAS

Managed by University of Georgia

Ali Missaoui – cssamm@uga.edu

The experimental sites were located at UGA Gibbs Farm in Tifton, GA. The study utilized a randomized complete block (RCB) design with three replications for each of the two treatments: drought-stressed (covered, CV) and control (uncovered, UC). Two replications for each treatment were established in August 2018, and the third replication for each treatment was established in April 2019. Biomass harvest was conducted in the first two replications only during the fall of 2019, while subsequent harvests were carried out in all three replications each year from 2020 to 2022. A total of 367 diverse accessions comprised the lowland, coastal, and upland switchgrass were used.

 

University of Tennessee – GWAS

Mitra Mazarei – mmazarei@utk.edu

A highly diverse switchgrass GWAS panel consisted of 330 accessions was planted under low and moderate nitrogen fertility treatments (two replicates per each nitrogen treatment) at Knoxville, TN (35.903094, −83.959253) in spring 2019. Genotypes were planted in a honeycomb design with 3.5 m spacing between plants. The field includes ‘Alamo’ AP13 as control/check genotype with 40 replicate plants (20 at low and 20 at moderate nitrogen). The field contains switchgrass ‘Blackwell’ buffer zone between nitrogen treatment plots and border. The field includes weed-cloth. The field area is 0.92 hectare.